Publication Year:
2017 2016

Publications from 2017

Beyersdorf MS, Sircar R, Lookadoo DB, Bottone CJ, Lynch MJ, Crane BR, Halkides CJ. Production, characterization, and assessment of a stable analog of the response regulator CheY-phosphate from Thermotoga maritima. Protein Sci. 2017 Apr 25. PubMed PMID: 28440031.

Sukomon N, Widom J, Borbat PP, Freed JH, Crane BR. Stability and Conformation of a Chemoreceptor HAMP Domain Chimera Correlates with Signaling Properties. Biophys J. 2017 Apr 11;112(7):1383-1395. PubMed PMID: 28402881.

Kim EA, Panushka J, Meyer T, Carlisle R, Baker S, Ide N, Lynch M, Crane BR, Blair DF. Architecture of the Flagellar Switch Complex of Escherichia coli: Conformational Plasticity of FliG and Implications for Adaptive Remodeling. J Mol Biol. 2017 May 5;429(9):1305-1320. PubMed PMID: 28259628.

Ganguly A, Thiel W, Crane BR. Glutamine Amide Flip Elicits Long Distance Allosteric Responses in the LOV Protein Vivid. J Am Chem Soc. 2017 Mar 1;139(8):2972-2980. PubMed PMID: 28145707.

Lynch MJ, Levenson R, Kim EA, Sircar R, Blair DF, Dahlquist FW, Crane BR. Co-Folding of a FliF-FliG Split Domain Forms the Basis of the MS:C Ring Interface within the Bacterial Flagellar Motor. Structure. 2017 Feb 7;25(2):317-328. PubMed PMID: 28089452.

Top of Page

Publications from 2016

Samanta D, Widom J, Borbat PP, Freed JH, Crane BR. Bacterial Energy Sensor Aer Modulates the Activity of the Chemotaxis Kinase CheA Based on the Redox State of the Flavin Cofactor. J Biol Chem. 2016 Dec 9;291(50):25809-25814. PubMed PMID: 27803157.

Miller MR, Miller KA, Bian J, James ME, Zhang S, Lynch MJ, Callery PS, Hettick JM, Cockburn A, Liu J, Li C, Crane BR, Charon NW. Spirochaete flagella hook proteins self-catalyse a lysinoalanine covalent crosslink for motility. Nat Microbiol. 2016 Aug 8;1(10):16134. doi: 10.1038/nmicrobiol.2016.134. PubMed PMID: 27670115.

Ganguly A, Manahan CC, Top D, Yee EF, Lin C, Young MW, Thiel W, Crane BR. Changes in active site histidine hydrogen bonding trigger cryptochrome activation. Proc Natl Acad Sci U S A. 2016 Sep 6;113(36):10073-8. doi: 10.1073/pnas.1606610113. PubMed PMID: 27551082.

Payne TM, Yee EF, Dzikovski B, Crane BR. Constraints on the Radical Cation Center of Cytochrome c Peroxidase for Electron Transfer from Cytochrome c. Biochemistry. 2016 Aug 30;55(34):4807-22. doi: 10.1021/acs.biochem.6b00262. PubMed PMID: 27499202.

Conrad KS, Hurley JM, Widom J, Ringelberg CS, Loros JJ, Dunlap JC, Crane BR. Structure of the frequency‐interacting RNA helicase: a protein interaction hub for the circadian clock. EMBO J. 2016 Jun 23. pii: e201694327. doi: 10.15252/embj.201694327. PubMed PMID: 27340124.

Top of Page

Publications from 2015

Yee EF, Diensthuber RP, Vaidya AT, Borbat PP, Engelhard C, Freed JH, Bittl R, Möglich A, Crane BR. Signal transduction in light-oxygen-voltage receptors lacking the adduct-forming cysteine residue. Nat Commun. 2015 Dec 9;6:10079. doi: 10.1038/ncomms10079. PubMed PMID: 26648256.

Greenswag AR, Muok A, Li X, Crane BR. Conformational Transitions that Enable Histidine Kinase Autophosphorylation and Receptor Array Integration. J Mol Biol. 2015 Dec 4;427(24):3890-907. doi: 10.1016/j.jmb.2015.10.015. Epub 2015 Oct 30. PubMed PMID: 26522934.

Greenswag AR, Li X, Borbat PP, Samanta D, Watts KJ, Freed JH, Crane BR. Preformed Soluble Chemoreceptor Trimers That Mimic Cellular Assembly States and Activate CheA Autophosphorylation. Biochemistry. 2015 Jun 9;54(22):3454-68. doi: 10.1021/bi501570n. Epub 2015 May 28. PubMed PMID: 25967982.

Sircar R, Borbat PP, Lynch MJ, Bhatnagar J, Beyersdorf MS, Halkides CJ, Freed JH, Crane BR. Assembly States of FliM and FliG within the Flagellar Switch Complex. J Mol Biol. 2015 Feb 27;427(4):867-86. doi: 10.1016/j.jmb.2014.12.009. Epub 2014 Dec 20. PubMed PMID: 25536293.

Samanta D, Borbat PP, Dzikovski B, Freed JH, Crane BR. Bacterial chemoreceptor dynamics correlate with activity state and are coupled over long distances. Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2455-60. doi: 10.1073/pnas.1414155112. Epub 2015 Feb 9. PubMed PMID: 25675479.

Top of Page

Publications from 2014

Pratt AJ, Shin DS, Merz GE, Rambo RP, Lancaster WA, Dyer KN, Borbat PP, Poole FL 2nd, Adams MW, Freed JH, Crane BR, Tainer JA, Getzoff ED. Aggregation propensities of superoxide dismutase G93 hotspot mutants mirror ALS clinical phenotypes. Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4568-76. doi: 10.1073/pnas.1308531111. Epub 2014 Oct 14. PubMed PMID: 25316790.

Davydov R, Labby KJ, Chobot SE, Lukoyanov DA, Crane BR, Silverman RB, Hoffman BM. Enzymatic and Cryoreduction EPR Studies of the Hydroxylation of Methylated Nω-Hydroxy-l-arginine Analogues by Nitric Oxide Synthase from Geobacillus stearothermophilus. Biochemistry. 2014 Oct 21;53(41):6511-9. doi: 10.1021/bi500485z. Epub 2014 Oct 8. PubMed PMID: 25251261.

Merz GE, Borbat PP, Pratt AJ, Getzoff ED, Freed JH, Crane BR. Copper-Based Pulsed Dipolar ESR Spectroscopy as a Probe of Protein Conformation Linked to Disease States. Biophys J. 2014 Oct 7;107(7):1669-74. doi: 10.1016/j.bpj.2014.07.068. PubMed PMID: 25296320.

Conrad KS, Manahan CC, Crane BR. Photochemistry of flavoprotein light sensors. Nat Chem Biol. 2014 Sep 17;10(10):801-809. doi: 10.1038/nchembio.1633. PubMed PMID: 25229449.

Crane BR, Young MW. Interactive features of proteins composing eukaryotic circadian clocks. Annu Rev Biochem. 2014;83:191-219. doi: 10.1146/annurev-biochem-060713-035644. PubMed PMID: 24905781.

Top of Page

Publications from 2013

Vaidya AT, Top D, Manahan CC, Tokuda JM, Zhang S, Pollack L, Young MW, Crane BR. Flavin reduction activates Drosophila cryptochrome. Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20455-60. doi: 10.1073/pnas.1313336110. Epub 2013 Dec 2. PubMed PMID: 24297896; PubMed Central PMCID: PMC3870761.

Jiang N, Kuznetsov A, Nocek JM, Hoffman BM, Crane BR, Hu X, Beratan DN. Distance-independent charge recombination kinetics in cytochrome c-cytochrome c peroxidase complexes: compensating changes in the electronic coupling and reorganization energies. J Phys Chem B. 2013 Aug 8;117(31):9129-41. doi: 10.1021/jp401551t. Epub 2013 Jul 29. PubMed PMID: 23895339; PubMed Central PMCID: PMC3809023.

Piasta KN, Ulliman CJ, Slivka PF, Crane BR, Falke JJ. Defining a key receptor-CheA kinase contact and elucidating its function in the membrane-bound bacterial chemosensory array: a disulfide mapping and TAM-IDS Study. Biochemistry. 2013 Jun 4;52(22):3866-80. doi: 10.1021/bi400385c. Epub 2013 May 23. PubMed PMID: 23668882; PubMed Central PMCID: PMC3705729.

Li X, Fleetwood AD, Bayas C, Bilwes AM, Ortega DR, Falke JJ, Zhulin IB, Crane BR. The 3.2 Å resolution structure of a receptor: CheA:CheW signaling complex defines overlapping binding sites and key residue interactions within bacterial chemosensory arrays. Biochemistry. 2013 Jun 4;52(22):3852-65. doi: 10.1021/bi400383e. Epub 2013 May 23. PubMed PMID: 23668907; PubMed Central PMCID: PMC3694592.

Sircar R, Greenswag AR, Bilwes AM, Gonzalez-Bonet G, Crane BR. Structure and activity of the flagellar rotor protein FliY: a member of the CheC phosphatase family. J Biol Chem. 2013 May 10;288(19):13493-502. doi: 10.1074/jbc.M112.445171. Epub 2013 Mar 26. PubMed PMID: 23532838; PubMed Central PMCID: PMC3650386.

Levy C, Zoltowski BD, Jones AR, Vaidya AT, Top D, Widom J, Young MW, Scrutton NS, Crane BR, Leys D. Updated structure of Drosophila cryptochrome. Nature. 2013 Mar 21;495(7441):E3-4. doi: 10.1038/nature11995. PubMed PMID: 23518567; PubMed Central PMCID: PMC3694752.

Airola MV, Huh D, Sukomon N, Widom J, Sircar R, Borbat PP, Freed JH, Watts KJ, Crane BR. Architecture of the soluble receptor Aer2 indicates an in-line mechanism for PAS and HAMP domain signaling. J Mol Biol. 2013 Mar 11;425(5):886-901. doi: 10.1016/j.jmb.2012.12.011. Epub 2012 Dec 26. PubMed PMID: 23274111; PubMed Central PMCID: PMC3577987.

Airola MV, Sukomon N, Samanta D, Borbat PP, Freed JH, Watts KJ, Crane BR. HAMP domain conformers that propagate opposite signals in bacterial chemoreceptors. PLoS Biol. 2013;11(2):e1001479. doi: 10.1371/journal.pbio.1001479. Epub 2013 Feb 12. PubMed PMID: 23424282; PubMed Central PMCID: PMC3570549.

Conrad KS, Bilwes AM, Crane BR. Light-induced subunit dissociation by a light-oxygen-voltage domain photoreceptor from Rhodobacter sphaeroides. Biochemistry. 2013 Jan 15;52(2):378-91. doi: 10.1021/bi3015373. Epub 2013 Jan 3. PubMed PMID: 23252338; PubMed Central PMCID: PMC3582384.

Top of Page

Publications from 2012

McLaughlin MP, Retegan M, Bill E, Payne TM, Shafaat HS, Peña S, Sudhamsu J, Ensign AA, Crane BR, Neese F, Holland PL. Azurin as a protein scaffold for a low-coordinate nonheme iron site with a small-molecule binding pocket. J Am Chem Soc. 2012 Dec 5;134(48):19746-57. doi: 10.1021/ja308346b. Epub 2012 Nov 20. PubMed PMID: 23167247; PubMed Central PMCID: PMC3515693.

Barry SM, Kers JA, Johnson EG, Song L, Aston PR, Patel B, Krasnoff SB, Crane BR, Gibson DM, Loria R, Challis GL. Cytochrome P450–catalyzed L-tryptophan nitration in thaxtomin phytotoxin biosynthesis. Nat Chem Biol. 2012 Oct;8(10):814-6. PubMed PMID: 22941045; PubMed Central PMCID: PMC3522571.

Crane BR. Biochemistry. Nature’s intricate clockwork. Science. 2012 Jul 13;337(6091):165-6. doi: 10.1126/science.1224611. PubMed PMID: 22798591.

Park S, Kim KY, Kim S, Crane BR. Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains in complex with CheW. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):713-5. doi: 10.1107/S174430911201826X. Epub 2012 May 24. PubMed PMID: 22684078; PubMed Central PMCID: PMC3370918.

Bhatnagar J, Sircar R, Borbat PP, Freed JH, Crane BR. Self-association of the histidine kinase CheA as studied by pulsed dipolar ESR spectroscopy. Biophys J. 2012 May 2;102(9):2192-201. doi: 10.1016/j.bpj.2012.03.038. PubMed PMID: 22824284; PubMed Central PMCID: PMC3341552.

Briegel A, Li X, Bilwes AM, Hughes KT, Jensen GJ, Crane BR. Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins. Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):3766-71. doi: 10.1073/pnas.1115719109. Epub 2012 Feb 21. PubMed PMID: 22355139; PubMed Central PMCID: PMC3309718.

Top of Page

Publications from 2011

Zoltowski BD, Vaidya AT, Top D, Widom J, Young MW, Crane BR. Structure of full-length Drosophila cryptochrome. Nature. 2011 Nov 13;480(7377):396-9. doi: 10.1038/nature10618. Erratum in: Nature. 2013 Apr 11;496(7444):252. PubMed PMID: 22080955; PubMed Central PMCID: PMC3240699.

Park S, Crane BR. Structural insight into the low affinity between Thermotoga maritima CheA and CheB compared to their Escherichia coli/Salmonella typhimurium counterparts. Int J Biol Macromol. 2011 Nov 1;49(4):794-800. doi: 10.1016/j.ijbiomac.2011.07.012. Epub 2011 Jul 23. PubMed PMID: 21816169; PubMed Central PMCID: PMC3204391.

King HA, Hoelz A, Crane BR, Young MW. Structure of an enclosed dimer formed by the Drosophila period protein. J Mol Biol. 2011 Oct 28;413(3):561-72. doi: 10.1016/j.jmb.2011.08.048. Epub 2011 Sep 3. PubMed PMID: 21907720; PubMed Central PMCID: PMC3252215.

Vaidya AT, Chen CH, Dunlap JC, Loros JJ, Crane BR. Structure of a light-activated LOV protein dimer that regulates transcription. Sci Signal. 2011 Aug 2;4(184):ra50. doi: 10.1126/scisignal.2001945. PubMed PMID: 21868352; PubMed Central PMCID: PMC3401549.

Cho KH, Crane BR, Park S. An insight into the interaction mode between CheB and chemoreceptor from two crystal structures of CheB methylesterase catalytic domain. Biochem Biophys Res Commun. 2011 Jul 22;411(1):69-75. doi: 10.1016/j.bbrc.2011.06.090. Epub 2011 Jun 21. PubMed PMID: 21722627; PubMed Central PMCID: PMC3158910.

Crane BR. Structural biology by mass spectrometry: mapping protein interaction surfaces of membrane receptor complexes with ICAT. J Mol Biol. 2011 Jun 17;409(4):481-2. doi: 10.1016/j.jmb.2011.04.030. Epub 2011 Apr 16. PubMed PMID: 21515283; PubMed Central PMCID: PMC3187936.

Paul K, Gonzalez-Bonet G, Bilwes AM, Crane BR, Blair D. Architecture of the flagellar rotor. EMBO J. 2011 Jun 14;30(14):2962-71. doi: 10.1038/emboj.2011.188. PubMed PMID: 21673656; PubMed Central PMCID: PMC3160249.

Park SY, Ham SW, Kim KY, Crane BR. Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli CheA P3 dimerization domain. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jun 1;67(Pt 6):662-4. doi: 10.1107/S1744309111010335. Epub 2011 May 25. PubMed PMID: 21636905; PubMed Central PMCID: PMC3107136.

Blanco-Rodríguez AM, Di Bilio AJ, Shih C, Museth AK, Clark IP, Towrie M, Cannizzo A, Sudhamsu J, Crane BR, Sýkora J, Winkler JR, Gray HB, Záliš S, Vlček A Jr. Phototriggering electron flow through Re(I)-modified Pseudomonas aeruginosa azurins. Chemistry. 2011 May 2;17(19):5350-61. doi: 10.1002/chem.201002162. Epub 2011 Apr 5. PubMed PMID: 21469225; PubMed Central PMCID: PMC3108028.

Park S, Crane BR. Crystallization and preliminary X-ray crystallographic analysis of CheW from Thermotoga maritima: a coupling protein of CheA and the chemotaxis receptor. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):504-6. doi: 10.1107/S1744309111005951. Epub 2011 Mar 30. PubMed PMID: 21505252; PubMed Central PMCID: PMC3080161.

Top of Page

Publications from 2010

Patel BA, Crane BR. When it comes to antibiotics, bacteria show some NO-how. J Mol Cell Biol. 2010 Oct;2(5):234-6. doi: 10.1093/jmcb/mjp044. Epub 2010 Jan 19. PubMed PMID: 20086004.

Sudhamsu J, Kabir M, Airola MV, Patel BA, Yeh SR, Rousseau DL, Crane BR. Co-expression of ferrochelatase allows for complete heme incorporation into recombinant proteins produced in E. coli. Protein Expr Purif. 2010 Sep;73(1):78-82. doi: 10.1016/j.pep.2010.03.010. Epub 2010 Mar 18. PubMed PMID: 20303407; PubMed Central PMCID: PMC2875318.

Airola MV, Watts KJ, Crane BR. Identifying divergent HAMP domains and poly-HAMP chains. J Biol Chem. 2010 Jun 4;285(23):le7; author reply 1e8. doi: 10.1074/jbc.L109.075721. PubMed PMID: 20511236; PubMed Central PMCID: PMC2878581.

Airola MV, Du J, Dawson JH, Crane BR. Heme binding to the Mammalian circadian clock protein period 2 is nonspecific. Biochemistry. 2010 May 25;49(20):4327-38. doi: 10.1021/bi901945w. PubMed PMID: 20411915; PubMed Central PMCID: PMC2873066.

Bhatnagar J, Borbat PP, Pollard AM, Bilwes AM, Freed JH, Crane BR. Structure of the ternary complex formed by a chemotaxis receptor signaling domain, the CheA histidine kinase, and the coupling protein CheW as determined by pulsed dipolar ESR spectroscopy. Biochemistry. 2010 May 11;49(18):3824-41. doi: 10.1021/bi100055m. PubMed PMID: 20355710; PubMed Central PMCID: PMC2873776.

Airola MV, Watts KJ, Bilwes AM, Crane BR. Structure of concatenated HAMP domains provides a mechanism for signal transduction. Structure. 2010 Mar 14;18(4):436-48. doi: 10.1016/j.str.2010.01.013. PubMed PMID: 20399181; PubMed Central PMCID: PMC2892831.

Kinloch RD, Sono M, Sudhamsu J, Crane BR, Dawson JH. Magnetic circular dichroism spectroscopic characterization of the NOS-like protein from Geobacillus stearothermophilus (gsNOS). J Inorg Biochem. 2010 Mar;104(3):357-64. doi: 10.1016/j.jinorgbio.2009.12.014. Epub 2010 Jan 4. PubMed PMID: 20110129.

Crane BR, Sudhamsu J, Patel BA. Bacterial nitric oxide synthases. Annu Rev Biochem. 2010;79:445-70. doi: 10.1146/annurev-biochem-062608-103436. Review. PubMed PMID: 20370423.

Top of Page

Publications from 2009

Lamb JS, Zoltowski BD, Pabit SA, Li L, Crane BR, Pollack L. Illuminating solution responses of a LOV domain protein with photocoupled small-angle X-ray scattering. J Mol Biol. 2009 Nov 6;393(4):909-19. doi: 10.1016/j.jmb.2009.08.045. Epub 2009 Aug 25. PubMed PMID: 19712683; PubMed Central PMCID: PMC2858630.

Zoltowski BD, Vaccaro B, Crane BR. Mechanism-based tuning of a LOV domain photoreceptor. Nat Chem Biol. 2009 Nov;5(11):827-34. doi: 10.1038/nchembio.210. Epub 2009 Aug 30. PubMed PMID: 19718042; PubMed Central PMCID: PMC2865183.

Patel BA, Moreau M, Widom J, Chen H, Yin L, Hua Y, Crane BR. Endogenous nitric oxide regulates the recovery of the radiation-resistant bacterium Deinococcus radiodurans from exposure to UV light. Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18183-8. doi: 10.1073/pnas.0907262106. Epub 2009 Oct 19. PubMed PMID: 19841256; PubMed Central PMCID: PMC2775278.

Davydov R, Sudhamsu J, Lees NS, Crane BR, Hoffman BM. EPR and ENDOR characterization of the reactive intermediates in the generation of NO by cryoreduced oxy-nitric oxide synthase from Geobacillus stearothermophilus. J Am Chem Soc. 2009 Oct 14;131(40):14493-507. doi: 10.1021/ja906133h. PubMed PMID: 19754116.

Blanco-Rodríguez AM, Busby M, Ronayne K, Towrie M, Grădinaru C, Sudhamsu J, Sýkora J, Hof M, Zális S, Di Bilio AJ, Crane BR, Gray HB, Vlcek A Jr. Relaxation dynamics of Pseudomonas aeruginosa Re(I)(CO)3(alpha-diimine)(HisX)+ (X = 83, 107, 109, 124, 126)Cu(II) azurins. J Am Chem Soc. 2009 Aug 26;131(33):11788-800. doi: 10.1021/ja902744s. PubMed PMID: 19639996.

Scharf BE, Aldridge PD, Kirby JR, Crane BR. Upward mobility and alternative lifestyles: a report from the 10th biennial meeting on Bacterial Locomotion and Signal Transduction. Mol Microbiol. 2009 Jul;73(1):5-19. doi: 10.1111/j.1365-2958.2009.06742.x. Epub 2009 Jun 1. PubMed PMID: 19496930; PubMed Central PMCID: PMC2724751.

Sudhamsu J, Crane BR. Bacterial nitric oxide synthases: what are they good for? Trends Microbiol. 2009 May;17(5):212-8. doi: 10.1016/j.tim.2009.02.003. Epub 2009 Apr 15. Review. PubMed PMID: 19375324.

Pollard AM, Bilwes AM, Crane BR. The structure of a soluble chemoreceptor suggests a mechanism for propagating conformational signals. Biochemistry. 2009 Mar 10;48(9):1936-44. doi: 10.1021/bi801727m. PubMed PMID: 19149470; PubMed Central PMCID: PMC2695681.

Hoke KR, Crane BR. The solution electrochemistry of tetrahydrobiopterin revisited. Nitric Oxide. 2009 Mar;20(2):79-87. doi: 10.1016/j.niox.2008.11.002. Epub 2008 Nov 27. PubMed PMID: 19059356.

Top of Page

Publications from 2008

Crane BR. The enzymology of nitric oxide in bacterial pathogenesis and resistance. Biochem Soc Trans. 2008 Dec;36(Pt 6):1149-54. doi: 10.1042/BST0361149. Review. PubMed PMID: 19021514.

Kabir M, Sudhamsu J, Crane BR, Yeh SR, Rousseau DL. Substrate-ligand interactions in Geobacillus stearothermophilus nitric oxide synthase. Biochemistry. 2008 Nov 25;47(47):12389-97. doi: 10.1021/bi801491e. PubMed PMID: 18956884; PubMed Central PMCID: PMC3403685.

Sudhamsu J, Lee GI, Klessig DF, Crane BR. The structure of YqeH. An AtNOS1/AtNOA1 ortholog that couples GTP hydrolysis to molecular recognition. J Biol Chem. 2008 Nov 21;283(47):32968-76. doi: 10.1074/jbc.M804837200. Epub 2008 Sep 18. PubMed PMID: 18801747; PubMed Central PMCID: PMC2583316.

Moreau M, Lee GI, Wang Y, Crane BR, Klessig DF. AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase. J Biol Chem. 2008 Nov 21;283(47):32957-67. doi: 10.1074/jbc.M804838200. Epub 2008 Sep 18. PubMed PMID: 18801746; PubMed Central PMCID: PMC2583314.

Garcin ED, Arvai AS, Rosenfeld RJ, Kroeger MD, Crane BR, Andersson G, Andrews G, Hamley PJ, Mallinder PR, Nicholls DJ, St-Gallay SA, Tinker AC, Gensmantel NP, Mete A, Cheshire DR, Connolly S, Stuehr DJ, Aberg A, Wallace AV, Tainer JA, Getzoff ED. Anchored plasticity opens doors for selective inhibitor design in nitric oxide synthase. Nat Chem Biol. 2008 Nov;4(11):700-7. doi: 10.1038/nchembio.115. Epub 2008 Oct 12. PubMed PMID: 18849972; PubMed Central PMCID: PMC2868503.

Lamb JS, Zoltowski BD, Pabit SA, Crane BR, Pollack L. Time-resolved dimerization of a PAS-LOV protein measured with photocoupled small angle X-ray scattering. J Am Chem Soc. 2008 Sep 17;130(37):12226-7. doi: 10.1021/ja804236f. Epub 2008 Aug 21. PubMed PMID: 18715002; PubMed Central PMCID: PMC2743002.

Zoltowski BD, Crane BR. Light activation of the LOV protein vivid generates a rapidly exchanging dimer. Biochemistry. 2008 Jul 8;47(27):7012-9. doi: 10.1021/bi8007017. Epub 2008 Jun 14. PubMed PMID: 18553928; PubMed Central PMCID: PMC2743001.

Shih C, Museth AK, Abrahamsson M, Blanco-Rodriguez AM, Di Bilio AJ, Sudhamsu J, Crane BR, Ronayne KL, Towrie M, Vlcek A Jr, Richards JH, Winkler JR, Gray HB. Tryptophan-accelerated electron flow through proteins. Science. 2008 Jun 27;320(5884):1760-2. doi: 10.1126/science.1158241. PubMed PMID: 18583608.

Johnson EG, Sparks JP, Dzikovski B, Crane BR, Gibson DM, Loria R. Plant-pathogenic Streptomyces species produce nitric oxide synthase-derived nitric oxide in response to host signals. Chem Biol. 2008 Jan;15(1):43-50. doi: 10.1016/j.chembiol.2007.11.014. PubMed PMID: 18215772.

Top of Page

Publications from 2007

Zoltowski BD, Schwerdtfeger C, Widom J, Loros JJ, Bilwes AM, Dunlap JC, Crane BR. Conformational switching in the fungal light sensor Vivid. Science. 2007 May 18;316(5827):1054-7. PubMed PMID: 17510367; PubMed Central PMCID: PMC3682417.

Wang ZQ, Lawson RJ, Buddha MR, Wei CC, Crane BR, Munro AW, Stuehr DJ. Bacterial flavodoxins support nitric oxide production by Bacillus subtilis nitric-oxide synthase. J Biol Chem. 2007 Jan 26;282(4):2196-202. Epub 2006 Nov 24. PubMed PMID: 17127770.

Zhang Y, Kang SA, Mukherjee T, Bale S, Crane BR, Begley TP, Ealick SE. Crystal structure and mechanism of tryptophan 2,3-dioxygenase, a heme enzyme involved in tryptophan catabolism and in quinolinate biosynthesis. Biochemistry. 2007 Jan 9;46(1):145-55. PubMed PMID: 17198384.

Bhatnagar J, Freed JH, Crane BR. Rigid body refinement of protein complexes with long-range distance restraints from pulsed dipolar ESR. Methods Enzymol. 2007;423:117-33. PubMed PMID: 17609128.

Top of Page

Publications from 2006

Gradinaru C, Crane BR. Comparison of intra- vs intermolecular long-range electron transfer in crystals of ruthenium-modified azurin. J Phys Chem B. 2006 Oct 19;110(41):20073-6. PubMed PMID: 17034174.

Park SY, Lowder B, Bilwes AM, Blair DF, Crane BR. Structure of FliM provides insight into assembly of the switch complex in the bacterial flagella motor. Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11886-91. Epub 2006 Aug 1. PubMed PMID: 16882724; PubMed Central PMCID: PMC1567671.

Park SY, Borbat PP, Gonzalez-Bonet G, Bhatnagar J, Pollard AM, Freed JH, Bilwes AM, Crane BR. Reconstruction of the chemotaxis receptor-kinase assembly. Nat Struct Mol Biol. 2006 May;13(5):400-7. Epub 2006 Apr 23. PubMed PMID: 16622408.

Sudhamsu J, Crane BR. Structure and reactivity of a thermostable prokaryotic nitric-oxide synthase that forms a long-lived oxy-heme complex. J Biol Chem. 2006 Apr 7;281(14):9623-32. Epub 2006 Jan 11. PubMed PMID: 16407211.

Blanco-Rodríguez AM, Busby M, Gradinaru C, Crane BR, Di Bilio AJ, Matousek P, Towrie M, Leigh BS, Richards JH, Vlcek A Jr, Gray HB. Excited-state dynamics of structurally characterized [ReI(CO)3(phen)(HisX)]+ (X = 83, 109) Pseudomonas aeruginosa azurins in aqueous solution. J Am Chem Soc. 2006 Apr 5;128(13):4365-70. PubMed PMID: 16569013.

Pant K, Crane BR. Nitrosyl-heme structures of Bacillus subtilis nitric oxide synthase have implications for understanding substrate oxidation. Biochemistry. 2006 Feb 28;45(8):2537-44. PubMed PMID: 16489746.

Kang SA, Hoke KR, Crane BR. Solvent isotope effects on interfacial protein electron transfer in crystals and electrode films. J Am Chem Soc. 2006 Feb 22;128(7):2346-55. PubMed PMID: 16478190.

Chao X, Muff TJ, Park SY, Zhang S, Pollard AM, Ordal GW, Bilwes AM, Crane BR. A receptor-modifying deamidase in complex with a signaling phosphatase reveals reciprocal regulation. Cell. 2006 Feb 10;124(3):561-71. PubMed PMID: 16469702.

Top of Page

Publications from 2005

Kang SA, Crane BR. Effects of interface mutations on association modes and electron-transfer rates between proteins. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15465-70. Epub 2005 Oct 14. PubMed PMID: 16227441; PubMed Central PMCID: PMC1266099.

Pant K, Crane BR. Structure of a loose dimer: an intermediate in nitric oxide synthase assembly. J Mol Biol. 2005 Sep 30;352(4):932-40. PubMed PMID: 16126221.

Buddha MR, Crane BR. Structures of tryptophanyl-tRNA synthetase II from Deinococcus radiodurans bound to ATP and tryptophan. Insight into subunit cooperativity and domain motions linked to catalysis. J Biol Chem. 2005 Sep 9;280(36):31965-73. Epub 2005 Jul 5. PubMed PMID: 15998643.

Quezada CM, Hamel DJ, Gradinaru C, Bilwes AM, Dahlquist FW, Crane BR, Simon MI. Structural and chemical requirements for histidine phosphorylation by the chemotaxis kinase CheA. J Biol Chem. 2005 Aug 26;280(34):30581-5. Epub 2005 Jun 30. PubMed PMID: 15994328.

Buddha MR, Crane BR. Structure and activity of an aminoacyl-tRNA synthetase that charges tRNA with nitro-tryptophan. Nat Struct Mol Biol. 2005 Mar;12(3):274-5. Epub 2005 Feb 20. PubMed PMID: 15723076.

Top of Page

Publications from 2004

Buddha MR, Tao T, Parry RJ, Crane BR. Regioselective nitration of tryptophan by a complex between bacterial nitric-oxide synthase and tryptophanyl-tRNA synthetase. J Biol Chem. 2004 Nov 26;279(48):49567-70. Epub 2004 Oct 5. PubMed PMID: 15466862.

Park SY, Chao X, Gonzalez-Bonet G, Beel BD, Bilwes AM, Crane BR. Structure and function of an unusual family of protein phosphatases: the bacterial chemotaxis proteins CheC and CheX. Mol Cell. 2004 Nov 19;16(4):563-74. PubMed PMID: 15546616.

Buddha MR, Keery KM, Crane BR. An unusual tryptophanyl tRNA synthetase interacts with nitric oxide synthase in Deinococcus radiodurans. Proc Natl Acad Sci U S A. 2004 Nov 9;101(45):15881-6. Epub 2004 Nov 1. PubMed PMID: 15520379; PubMed Central PMCID: PMC528745.

Kang SA, Marjavaara PJ, Crane BR. Electron transfer between cytochrome c and cytochome c peroxidase in single crystals. J Am Chem Soc. 2004 Sep 8;126(35):10836-7. PubMed PMID: 15339156.

Quezada CM, Gradinaru C, Simon MI, Bilwes AM, Crane BR. Helical shifts generate two distinct conformers in the atomic resolution structure of the CheA phosphotransferase domain from Thermotoga maritima. J Mol Biol. 2004 Aug 27;341(5):1283-94. PubMed PMID: 15321722.

Park SY, Beel BD, Simon MI, Bilwes AM, Crane BR. In different organisms, the mode of interaction between two signaling proteins is not necessarily conserved. Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11646-51. Epub 2004 Aug 2. PubMed PMID: 15289606; PubMed Central PMCID: PMC511033.

Kers JA, Wach MJ, Krasnoff SB, Widom J, Cameron KD, Bukhalid RA, Gibson DM, Crane BR, Loria R. Nitration of a peptide phytotoxin by bacterial nitric oxide synthase. Nature. 2004 May 6;429(6987):79-82. PubMed PMID: 15129284.

Wang ZQ, Wei CC, Sharma M, Pant K, Crane BR, Stuehr DJ. A conserved Val to Ile switch near the heme pocket of animal and bacterial nitric-oxide synthases helps determine their distinct catalytic profiles. J Biol Chem. 2004 Apr 30;279(18):19018-25. Epub 2004 Feb 19. PubMed PMID: 14976216.

Park SY, Quezada CM, Bilwes AM, Crane BR. Subunit exchange by CheA histidine kinases from the mesophile Escherichia coli and the thermophile Thermotoga maritima. Biochemistry. 2004 Mar 2;43(8):2228-40. PubMed PMID: 14979719.

Top of Page

Publications from 2003

Miller JE, Grădinaru C, Crane BR, Di Bilio AJ, Wehbi WA, Un S, Winkler JR, Gray HB. Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment. J Am Chem Soc. 2003 Nov 26;125(47):14220-1. PubMed PMID: 14624538.

Wei CC, Crane BR, Stuehr DJ. Tetrahydrobiopterin radical enzymology. Chem Rev. 2003 Jun;103(6):2365-83. Review. PubMed PMID: 12797834.

Top of Page

Publications from 2002

Pant K, Bilwes AM, Adak S, Stuehr DJ, Crane BR. Structure of a nitric oxide synthase heme protein from Bacillus subtilis. Biochemistry. 2002 Sep 17;41(37):11071-9. PubMed PMID: 12220171.

Tezcan FA, Findley WM, Crane BR, Ross SA, Lyubovitsky JG, Gray HB, Winkler JR. Using deeply trapped intermediates to map the cytochrome c folding landscape. Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8626-30. PubMed PMID: 12084923; PubMed Central PMCID: PMC124336.

Adak S, Bilwes AM, Panda K, Hosfield D, Aulak KS, McDonald JF, Tainer JA, Getzoff ED, Crane BR, Stuehr DJ. Cloning, expression, and characterization of a nitric oxide synthase protein from Deinococcus radiodurans. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):107-12. Epub 2001 Dec 26. PubMed PMID: 11756668; PubMed Central PMCID: PMC117522.

Dmochowski IJ, Dunn AR, Wilker JJ, Crane BR, Green MT, Dawson JH, Sligar SG, Winkler JR, Gray HB. Sensitizer-linked substrates and ligands: ruthenium probes of cytochrome P450 structure and mechanism. Methods Enzymol. 2002;357:120-33. PubMed PMID: 12424904.

Top of Page

Publications from 2001

Crane BR, Di Bilio AJ, Winkler JR, Gray HB. Electron tunneling in single crystals of Pseudomonas aeruginosa azurins. J Am Chem Soc. 2001 Nov 28;123(47):11623-31. PubMed PMID: 11716717.

Dunn AR, Dmochowski IJ, Bilwes AM, Gray HB, Crane BR. Probing the open state of cytochrome P450cam with ruthenium-linker substrates. Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12420-5. Epub 2001 Oct 16. PubMed PMID: 11606730; PubMed Central PMCID: PMC60069.

Ghosh DK, Rashid MB, Crane B, Taskar V, Mast M, Misukonis MA, Weinberg JB, Eissa NT. Characterization of key residues in the subdomain encoded by exons 8 and 9 of human inducible nitric oxide synthase: a critical role for Asp-280 in substrate binding and subunit interactions. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10392-7. Epub 2001 Aug 21. PubMed PMID: 11517317; PubMed Central PMCID:

Tezcan FA, Crane BR, Winkler JR, Gray HB. Electron tunneling in protein crystals. Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5002-6. Epub 2001 Apr 10. PubMed PMID: 11296248; PubMed Central PMCID: PMC33153.

Di Bilio AJ, Crane BR, Wehbi WA, Kiser CN, Abu-Omar MM, Carlos RM, Richards JH, Winkler JR, Gray HB. Properties of photogenerated tryptophan and tyrosyl radicals in structurally characterized proteins containing rhenium(I) tricarbonyl diimines. J Am Chem Soc. 2001 Apr 4;123(13):3181-2. PubMed PMID: 11457048.

Bilwes AM, Quezada CM, Croal LR, Crane BR, Simon MI. Nucleotide binding by the histidine kinase CheA. Nat Struct Biol. 2001 Apr;8(4):353-60. PubMed PMID: 11276258.

Top of Page

Publications from 2000

Crane BR, Arvai AS, Ghosh S, Getzoff ED, Stuehr DJ, Tainer JA. Structures of the N(omega)-hydroxy-L-arginine complex of inducible nitric oxide synthase oxygenase dimer with active and inactive pterins. Biochemistry. 2000 Apr 25;39(16):4608-21. PubMed PMID: 10769116.

Top of Page

Publications from 1999

Crane BR, Rosenfeld RJ, Arvai AS, Ghosh DK, Ghosh S, Tainer JA, Stuehr DJ, Getzoff ED. N-terminal domain swapping and metal ion binding in nitric oxide synthase dimerization. EMBO J. 1999 Nov 15;18(22):6271-81. PubMed PMID: 10562539; PubMed Central PMCID: PMC1171690.

Ghosh DK, Crane BR, Ghosh S, Wolan D, Gachhui R, Crooks C, Presta A, Tainer JA, Getzoff ED, Stuehr DJ. Inducible nitric oxide synthase: role of the N-terminal beta-hairpin hook and pterin-binding segment in dimerization and tetrahydrobiopterin interaction. EMBO J. 1999 Nov 15;18(22):6260-70. PubMed PMID: 10562538; PubMed Central PMCID: PMC1171689.

Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB. Optical detection of cytochrome P450 by sensitizer-linked substrates. Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12987-90. PubMed PMID: 10557259; PubMed Central PMCID: PMC23886.

Adak S, Crooks C, Wang Q, Crane BR, Tainer JA, Getzoff ED, Stuehr DJ. Tryptophan 409 controls the activity of neuronal nitric-oxide synthase by regulating nitric oxide feedback inhibition. J Biol Chem. 1999 Sep 17;274(38):26907-11. PubMed PMID: 10480900.

Ghosh S, Wolan D, Adak S, Crane BR, Kwon NS, Tainer JA, Getzoff ED, Stuehr DJ. Mutational analysis of the tetrahydrobiopterin-binding site in inducible nitric-oxide synthase. J Biol Chem. 1999 Aug 20;274(34):24100-12. PubMed PMID: 10446182.

Faham S, Day MW, Connick WB, Crane BR, Di Bilio AJ, Schaefer WP, Rees DC, Gray HB. Structures of ruthenium-modified Pseudomonas aeruginosa azurin and [Ru(2,2′-bipyridine)2(imidazole)2]SO4 x 10H2O. Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):379-85. PubMed PMID: 10089343.

Bilwes AM, Alex LA, Crane BR, Simon MI. Structure of CheA, a signal-transducing histidine kinase. Cell. 1999 Jan 8;96(1):131-41. PubMed PMID: 9989504.

Top of Page

Publications from 1998

Crane BR, Arvai AS, Ghosh DK, Wu C, Getzoff ED, Stuehr DJ, Tainer JA. Structure of nitric oxide synthase oxygenase dimer with pterin and substrate. Science. 1998 Mar 27;279(5359):2121-6. PubMed PMID: 9516116.

Top of Page

Publications from 1997

Crane BR, Arvai AS, Gachhui R, Wu C, Ghosh DK, Getzoff ED, Stuehr DJ, Tainer JA. The structure of nitric oxide synthase oxygenase domain and inhibitor complexes. Science. 1997 Oct 17;278(5337):425-31. PubMed PMID: 9334294.

Crane BR, Siegel LM, Getzoff ED. Probing the catalytic mechanism of sulfite reductase by X-ray crystallography: structures of the Escherichia coli hemoprotein in complex with substrates, inhibitors, intermediates, and products. Biochemistry. 1997 Oct 7;36(40):12120-37. PubMed PMID: 9315849.

Crane BR, Siegel LM, Getzoff ED. Structures of the siroheme- and Fe4S4-containing active center of sulfite reductase in different states of oxidation: heme activation via reduction-gated exogenous ligand exchange. Biochemistry. 1997 Oct 7;36(40):12101-19. PubMed PMID: 9315848.

Gachhui R, Ghosh DK, Wu C, Parkinson J, Crane BR, Stuehr DJ. Mutagenesis of acidic residues in the oxygenase domain of inducible nitric-oxide synthase identifies a glutamate involved in arginine binding. Biochemistry. 1997 Apr 29;36(17):5097-103. PubMed PMID: 9136868.

Crane BR, Getzoff ED. Determining phases and anomalous-scattering models from the multiwavelength anomalous diffraction of native protein metal clusters. improved MAD phase error estimates and anomalous-scatterer positions. Acta Crystallogr D Biol Crystallogr. 1997 Jan 1;53(Pt 1):23-40. PubMed PMID: 15299969.

Crane BR, Bellamy H, Getzoff ED. Multiwavelength anomalous diffraction of sulfite reductase hemoprotein: making the most of MAD data. Acta Crystallogr D Biol Crystallogr. 1997 Jan 1;53(Pt 1):8-22. PubMed PMID: 15299968.

Top of Page

Publications from 1996

Crane BR, Getzoff ED. The relationship between structure and function for the sulfite reductases. Curr Opin Struct Biol. 1996 Dec;6(6):744-56. Review. PubMed PMID: 8994874.

Ogihara NL, Parge HE, Hart PJ, Weiss MS, Goto JJ, Crane BR, Tsang J, Slater K, Roe JA, Valentine JS, Eisenberg D, Tainer JA. Unusual trigonal-planar copper configuration revealed in the atomic structure of yeast copper-zinc superoxide dismutase. Biochemistry. 1996 Feb 20;35(7):2316-21. PubMed PMID: 8652572.

Top of Page

Publications from 1995

Crane BR, Siegel LM, Getzoff ED. Sulfite reductase structure at 1.6 A: evolution and catalysis for reduction of inorganic anions. Science. 1995 Oct 6;270(5233):59-67. PubMed PMID: 7569952.

Top of Page

Publications from 1992

Kurz LC, Shah S, Crane BR, Donald LJ, Duckworth HW, Drysdale GR. Proton uptake accompanies formation of the ternary complex of citrate synthase, oxaloacetate, and the transition-state analog inhibitor, carboxymethyl-CoA. Evidence that a neutral enol is the activated form of acetyl-CoA in the citrate synthase reaction. Biochemistry. 1992 Sep 1;31(34):7899-907. PubMed PMID: 1324722.

Top of Page

Publications from 1991

Donald LJ, Crane BR, Anderson DH, Duckworth HW. The role of cysteine 206 in allosteric inhibition of Escherichia coli citrate synthase. Studies by chemical modification, site-directed mutagenesis, and 19F NMR. J Biol Chem. 1991 Nov 5;266(31):20709-13. PubMed PMID: 1939121.